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https://dipositint.ub.edu/dspace/handle/2445/160957
Title: | Bacterial cellulose matrices to develop enzymatically active paper |
Author: | Buruaga Ramiro, Carolina Valls Vidal, Cristina Valenzuela Mayorga, Susana Valeria Roncero, M. Blanca Pastor Blasco, Francisco I. Javier Díaz Lucea, Pilar Martínez Martínez, Josefina |
Keywords: | Cel·lulosa Bacteris Cellulose Bacteria |
Issue Date: | 31-Jan-2020 |
Publisher: | Springer Verlag |
Abstract: | This work studies the suitability of bacterial cellulose (BC) matrices to prepare enzymatically active nanocomposites, in a framework of more environmentally friendly methodologies. After BC production and purification, two kind of matrices were obtained: BC in aqueous suspension and BC paper. A lipase was immobilised onto the BC matrices by physical adsorption, obtaining Lipase/BC nanocomposites. Neither morphology nor crystallinity, measured by scanning electron microscopy (SEM) and X-Ray diffractometry (XRD) respectively, of the BC were affected by the binding of the protein. The activity of Lipase/BC suspension and Lipase/BC paper was tested under different conditions, and the operational properties of the enzyme were evaluated. A shift towards higher temperatures, a broader pH activity range, and slight differences in the substrate preference were observed in the immobilised lipase, compared with the free enzyme. Specific activity was higher for Lipase/BC suspension (4.2 U/mg) than for Lipase/BC paper (1.7 U/mg) nanocomposites. However, Lipase/BC paper nanocomposites showed improved thermal stability, reusability, and durability. Enzyme immobilised onto BC paper retained 60% of its activity after 48 h at 60 ºC. It maintained 100% of the original activity after being recycled 10 times at pH 7 at 60 ºC and it remained active after being stored for more than a month at room temperature. The results suggested that lipase/BC nanocomposites are promising biomaterials for the development of green biotechnological devices with potential application in industrials bioprocesses of detergents and food industry and biomedicine. Lipase/BC paper nanocomposite might be a key component of bioactive paper for developing simple, handheld, and disposable devices. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1007/s10570-020-03025-9 |
It is part of: | Cellulose, 2020, vol. 27, p. 3413-3426 |
URI: | http://hdl.handle.net/2445/160957 |
Related resource: | https://doi.org/10.1007/s10570-020-03025-9 |
ISSN: | 0969-0239 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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