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Title: | Ancestral roles of IĸB proteins in Caenorhabditis elegans |
Author: | Ahumada Brena, David Ricardo |
Director/Tutor: | Cerón Madrigal, Julián |
Keywords: | Epigenètica Càncer Proteïnes supressores de tumors Models animals en la investigació Epigenetics Cancer Tumor suppressor protein Animal models in research |
Issue Date: | 9-Jul-2021 |
Publisher: | Universitat de Barcelona |
Abstract: | [eng] Mammalian Inhibitor-of-kappa-B (IκBs) proteins exert their main function as negative regulators of NF-κB, a central signaling pathway controlling immunity and inflammation. An alternative chromatin role for IκBs has been shown to affect stemness and cell differentiation. However, the involvement of NF-κB in this function has not been excluded. NFKI-1 and IKB1 are IκB homologs in aenorhabditis elegans, which lacks NF-κB nuclear effectors. We found that nfki-1 and ikb-1 mutants display developmental defects that phenocopy mutations in Polycomb and UTX-1 histone demethylase, suggesting a role for C. elegans IκBs in chromatin regulation. Further supporting this possibility (i) we detected NFKI-1 in the nucleus of cells; (ii) NFKI-1 and IKB-1 bind to histones and Polycomb proteins, (iii) and associate with chromatin in vivo, and (iv) mutations in nfki-1 and ikb-1 alter chromatin marks. Based on these results, we propose that ancestral IκB proteins modulate chromatin marks with an impact on development. |
URI: | http://hdl.handle.net/2445/200760 |
Appears in Collections: | Tesis Doctorals - Facultat - Medicina |
Files in This Item:
File | Description | Size | Format | |
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DRAB_PhD_THESIS.pdf | 4.53 MB | Adobe PDF | View/Open |
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