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https://dipositint.ub.edu/dspace/handle/2445/201833
Title: | Arabidopsis metacaspase MC1 localizes in stress granules, clears protein aggregates and delays senescence |
Author: | Ruiz-Solaní, Nerea Salguero-Linares, Jose Armengot, Laia Santos, Jaime Pallarès, Irantzu van Midden, Katarina P. Phukkan, Ujjal J. Koyuncu, Seda Borràs-Bisa, Júlia Liang, Li Popa, Crina Eisele, Frederik Eisele-Bürger, Anna Maria Hill, Sandra Malgrem Gutiérrez-Beltrán, Emilio Nyström, Thomas Valls i Matheu, Marc Llamas, Ernesto Vilchez, David Klemenč, Marina Ventura, Salvador Coll, Nuria S. |
Keywords: | Arabidopsis Metabolisme cel·lular Genètica Arabidopsis Cell metabolism Genetics |
Issue Date: | 1-Jul-2023 |
Publisher: | American Society of Plant Physiologists |
Abstract: | Stress granules (SGs) are highly conserved cytoplasmic condensates that assemble in response to stress and contribute to maintaining protein homeostasis. These membraneless organelles are dynamic, disassembling once the stress is no longer present. Persistence of SGs due to mutations or chronic stress has been often related to age-dependent protein-misfolding diseases in animals. Here, we find that the metacaspase MC1 is dynamically recruited into SGs upon proteotoxic stress in Arabidopsis (Arabidopsis thaliana). Two predicted disordered regions, the prodomain and the 360 loop, mediate MC1 recruitment to and release from SGs. Importantly, we show that MC1 has the capacity to clear toxic protein aggregates in vivo and in vitro, acting as a disaggregase. Finally, we demonstrate that overexpressing MC1 delays senescence and this phenotype is dependent on the presence of the 360 loop and an intact catalytic domain. Together, our data indicate that MC1 regulates senescence through its recruitment into SGs and this function could potentially be linked to its remarkable protein aggregate-clearing activity. |
Note: | Reproducció del document publicat a: https://doi.org/10.1093/plcell/koad172 |
It is part of: | Plant Cell, 2023, vol. 35, num. 9, p. 3325-3344 |
URI: | http://hdl.handle.net/2445/201833 |
Related resource: | https://doi.org/10.1093/plcell/koad172 |
ISSN: | 1040-4651 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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739112.pdf | 6.67 MB | Adobe PDF | View/Open |
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