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DC Field | Value | Language |
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dc.contributor.author | Potts, Jordan C. | - |
dc.contributor.author | Jain, Akhil | - |
dc.contributor.author | Amabilino, David B. | - |
dc.contributor.author | Pérez García, M. Lluïsa (Maria Lluïsa) | - |
dc.contributor.author | Rawson, Frankie J. | - |
dc.date.accessioned | 2024-02-26T13:09:56Z | - |
dc.date.available | 2024-02-26T13:09:56Z | - |
dc.date.issued | 2023 | - |
dc.identifier.issn | 2573-2293 | - |
dc.identifier.uri | http://hdl.handle.net/2445/208055 | - |
dc.description.abstract | <p>Cytochrome c (Cyt c), known for its functional redox capabilities, plays a pivotal role in biological</p><p>processes such as the electron transport chain and apoptosis. However, understanding how different</p><p>conjugation strategies impact its structural and redox characteristics is limited. To fill this gap, we</p><p>investigated the effects of conjugating Cyt c and a zinc(II) porphyrin (Zn Porph) to gold nanoparticles</p><p>(AuNPs). We used circular dichroism (CD) spectroscopy to detect structural conformational changes</p><p>in Cyt c upon conjugation and time-of-flight secondary ion mass spectrometry (TOF-SIMS) to</p><p>identify protein orientation. Cyt c was predicted to have different orientations depending on the size</p><p>of AuNPs and methods used to conjugate the protein, it was hypothesised that the orientation of Cyt</p><p>https://doi.org/10.26434/chemrxiv-2023-rsrwv ORCID: https://orcid.org/0000-0002-4872-8928 Content not peer-reviewed by ChemRxiv. License: CC BY 4.0</p><p>2</p><p>c may influence the redox properties of the protein. The electrochemical properties of Cyt c were</p><p>assessed using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). We used DPVbased</p><p>to determine the heterogeneous rate constant (k0). The results show a lower k0 for conjugated</p><p>Cyt c than free Cyt c, likely due to structural changes in the protein. The spatial orientation of Cyt c</p><p>had minimal influence on k0, while ligand density and AuNP size had an effect. The k0 value of Zn</p><p>Porph did not decrease on conjugation. Despite these changes, Cyt c and Zn Porph maintained their</p><p>electrochemical capabilities after conjugation.</p> | - |
dc.format.extent | 1 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.relation.isformatof | https://doi.org/https://doi.org/10.26434/chemrxiv-2023-rsrwv | - |
dc.relation.ispartof | 2023 | - |
dc.relation.uri | https://doi.org/https://doi.org/10.26434/chemrxiv-2023-rsrwv | - |
dc.rights | , 2023 | - |
dc.source | Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica) | - |
dc.subject.classification | Toxicologia | - |
dc.subject.classification | Nanopartícules | - |
dc.subject.other | Toxicology | - |
dc.subject.other | Nanoparticles | - |
dc.title | Electrochemical analysis of gold nanoparticles multifunctionalised withCytochrome c and a zinc Porphyrin | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/ | - |
dc.identifier.idgrec | 744243 | - |
dc.date.updated | 2024-02-26T13:09:56Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica) |
Files in This Item:
File | Description | Size | Format | |
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846976.pdf | 3.77 MB | Adobe PDF | View/Open |
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