Please use this identifier to cite or link to this item: https://dipositint.ub.edu/dspace/handle/2445/209208
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dc.contributor.authorAlmacellas i Canals, Eugènia-
dc.contributor.authorGarcía Cajide, Marta-
dc.contributor.authorTauler Girona, Albert-
dc.contributor.authorMauvezin, Caroline-
dc.date.accessioned2024-03-26T17:40:22Z-
dc.date.issued2022-01-01-
dc.identifier.isbn978-1-0716-2071-7-
dc.identifier.urihttps://hdl.handle.net/2445/209208-
dc.description.abstractThe detection of autophagic vesicles in interphase cells is well characterized with markers such as LC3, SQSTM1 (also known as p62) and LAMP2, which are commonly used in immunofluorescence and biochemistry assays to evaluate the status of autophagy in adherent cells. During mitosis, cells undergo important morphological changes which alter the position of the central plane, therefore the imaging on dividing cells has to be specifically designed. Here, we describe a method to label and image autophagic vesicles in mitotic cells to systematically analyze their number, morphology and distribution.ca
dc.format.extent11 p.-
dc.format.mimetypeapplication/pdf-
dc.language.isoengca
dc.relation.isformatofVersió postprint del capítol del llibre publicat a: https://doi.org/10.1007/978-1-0716-2071-7_9-
dc.relation.ispartofAutophagy and Cancer. Methods in Molecular Biology, vol 2445, Norberg, H., Norberg, E. (eds), ISBN: 978-1-0716-2071-7, p. 127-137-
dc.relation.urihttps://doi.org/10.1007/978-1-0716-2071-7_9-
dc.rights(c) Almacellas i Canals et al., 2022-
dc.sourceLlibres / Capítols de llibre (Bioquímica i Biomedicina Molecular)-
dc.subject.classificationLisosomes-
dc.subject.classificationAutofàgia-
dc.subject.otherLysosomes-
dc.subject.otherAutophagy-
dc.titleAnalysis of autophagic vesicles in mitotic cellsca
dc.typeinfo:eu-repo/semantics/bookPartca
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessca
Appears in Collections:Llibres / Capítols de llibre (Bioquímica i Biomedicina Molecular)

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