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DC Field | Value | Language |
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dc.contributor.author | Ferrándiz, Nuria | - |
dc.contributor.author | Caraballo, Juan M. | - |
dc.contributor.author | García-Gutierrez, Lucia | - |
dc.contributor.author | Devgan, Vikram | - |
dc.contributor.author | Rodríguez-Paredes, Manuel | - |
dc.contributor.author | Lafita, M. Carmen | - |
dc.contributor.author | Bretones, Gabriel | - |
dc.contributor.author | Quintanilla, Andrea | - |
dc.contributor.author | Muñoz-Alonso, M. José | - |
dc.contributor.author | Blanco, Rosa | - |
dc.contributor.author | Reyes, José C. | - |
dc.contributor.author | Agell i Jané, Neus | - |
dc.contributor.author | Delgado, M. Dolores | - |
dc.contributor.author | Dotto, G. Paolo | - |
dc.contributor.author | León, Javier | - |
dc.date.accessioned | 2013-02-05T13:32:30Z | - |
dc.date.available | 2013-02-05T13:32:30Z | - |
dc.date.issued | 2012-05-25 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2445/33727 | - |
dc.description.abstract | It has been previously described that p21 functions not only as a CDK inhibitor but also as a transcriptional co-repressor in some systems. To investigate the roles of p21 in transcriptional control, we studied the gene expression changes in two human cell systems. Using a human leukemia cell line (K562) with inducible p21 expression and human primary keratinocytes with adenoviral-mediated p21 expression, we carried out microarray-based gene expression profiling. We found that p21 rapidly and strongly repressed the mRNA levels of a number of genes involved in cell cycle and mitosis. One of the most strongly down-regulated genes was CCNE2 (cyclin E2 gene). Mutational analysis in K562 cells showed that the N-terminal region of p21 is required for repression of gene expression of CCNE2 and other genes. Chromatin immunoprecipitation assays indicated that p21 was bound to human CCNE2 and other p21-repressed genes gene in the vicinity of the transcription start site. Moreover, p21 repressed human CCNE2 promoter-luciferase constructs in K562 cells. Bioinformatic analysis revealed that the CDE motif is present in most of the promoters of the p21-regulated genes. Altogether, the results suggest that p21 exerts a repressive effect on a relevant number of genes controlling S phase and mitosis. Thus, p21 activity as inhibitor of cell cycle progression would be mediated not only by the inhibition of CDKs but also by the transcriptional down-regulation of key genes. | - |
dc.format.extent | 13 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0037759 | - |
dc.relation.ispartof | PLoS One, 2012, vol. 7, num. 5, p. e37759 | - |
dc.relation.uri | http://dx.doi.org/10.1371/journal.pone.0037759 | - |
dc.rights | cc-by (c) Ferrándiz, N. et al., 2012 | - |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es | - |
dc.source | Articles publicats en revistes (Biomedicina) | - |
dc.subject.classification | Mitosi | - |
dc.subject.classification | Bioinformàtica | - |
dc.subject.classification | Expressió gènica | - |
dc.subject.other | Mitosis | - |
dc.subject.other | Bioinformatics | - |
dc.subject.other | Gene expression | - |
dc.title | p21 as a Transcriptional Co-Repressor of S-Phase and Mitotic Control Genes | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 614382 | - |
dc.date.updated | 2013-02-05T13:32:30Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
dc.identifier.pmid | 22662213 | - |
Appears in Collections: | Articles publicats en revistes (Biomedicina) |
Files in This Item:
File | Description | Size | Format | |
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614382.pdf | 1.17 MB | Adobe PDF | View/Open |
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