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DC Field | Value | Language |
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dc.contributor.author | Padilha, Itácio Q. M. | - |
dc.contributor.author | Valenzuela Mayorga, Susana Valeria | - |
dc.contributor.author | Grisi, Teresa | - |
dc.contributor.author | Díaz Lucea, Pilar | - |
dc.contributor.author | de Araújo, Demetrius | - |
dc.contributor.author | Pastor Blasco, Francisco I. Javier | - |
dc.date.accessioned | 2015-01-19T13:03:35Z | - |
dc.date.available | 2015-01-19T13:03:35Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1139-6709 | - |
dc.identifier.uri | http://hdl.handle.net/2445/61481 | - |
dc.description.abstract | A new xylanolytic strain, Paenibacillus favisporus CC02-N2, was isolated from sugarcane plantation fi elds in Brazil. The strain had a xylan-degrading system with multiple enzymes, one of which, xylanase Xyn30A, was identifi ed and characterized. The enzyme is a single-domain xylanase belonging to family 30 of the glycosyl hydrolases (GH30). Xyn30A shows high activity on glucuronoxylans, with a Vmax of 267.2 U mg<br>1, a Km of 4.0 mg/ml, and a kcat of 13,333 min<br>1 on beechwood xylan, but it does not hydrolyze arabinoxylans. The three- dimensional structure of Xyn30A consists of a common (β/α)8 barrel linked to a side-chain-associated β-structure, similar to previously characterized GH30 xylanases. The hydrolysis products from glucuronoxylan were methylglucuronic-acid-substituted xylooligomers (acidic xylooligosaccharides). The enzyme bound to insoluble xylan but not to crystalline cellulose. Our results suggest a specifi c role for Xyn30A in xylan biodegradation in natural habitats. The enzyme is a good candidate for the production of tailored xylooligosaccharides for use in the food industry and in the biotechnological transformation of biomass. [Int Microbiol 2014; 17(3):175-184] | - |
dc.format.extent | 10 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Spanish Society for Microbiology (SEM) and Viguera Editores SL | - |
dc.relation.isformatof | Reproducció del document publicat a: http://dx.doi.org/:10.2436/20.1501.01.220. | - |
dc.relation.ispartof | International Microbiology, 2014, vol. 17, num. 3, p. 175-184 | - |
dc.relation.uri | http://dx.doi.org/10.2436/20.1501.01.220. | - |
dc.rights | cc-by-nc-sa (c) Spanish Society for Microbiology (SEM) and Viguera Editores SL, 2014 | - |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/es | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Bacils | - |
dc.subject.classification | Ecologia microbiana | - |
dc.subject.classification | Enzims microbians | - |
dc.subject.classification | Biopolímers | - |
dc.subject.classification | Sòls | - |
dc.subject.other | Bacillus (Bacteria) | - |
dc.subject.other | Microbial ecology | - |
dc.subject.other | Microbial enzymes | - |
dc.subject.other | Biopolymers | - |
dc.subject.other | Soils | - |
dc.title | A glucuronoxylan-specific xylanase from a new Paenibacillus favisporus strain isolated from tropical soil of Brazil | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/publishedVersion | - |
dc.identifier.idgrec | 645915 | - |
dc.date.updated | 2015-01-19T13:03:35Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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